Desharnais was replaced by center Brian Flynn, who has not played since Game 5 of the Canadiens first-round series against the Ottawa Senators on April 24.
In seven games in the Stanley Cup Playoffs, Desharnais has no goals, two assists and a minus-2 rating.
Flynn had a goal and two assists in Game 1 against the Senators, but went without a point and was a minus-3 in his next four games.
The Lightning lead the best-of-7 series 1-0.
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